Fig. 6: Olfm2-null adipose transcriptomes show impaired fat cell cycle.
From: Defective Olfactomedin-2 connects adipocyte dysfunction to obesity
a We ran the transcriptomic analysis of epigonadal omental (OM) and inguinal subcutaneous (SC) adipose tissues of 10 diet-induced obese (DIO) Olfm2-null (Olfm2_KO) and wild-type (Wt) mice. Principal component analysis (PCA) shows b OM and c SC sample distribution in two-dimensional charts. Correlation plots picture transcript changes in d OM and e SC adipose tissues. Inked dots depict genes with FDR p < 0.05 and FC ≥ 1.2 (red) or FC ≤ −1.2 (blue), and green dots show genes related to the G2M checkpoint hallmark. GSEA summarizes the hallmarks affected in f OM and g SC fat. h Genes ascribed to G2M checkpoint and/or being E2F targets were broadly compromised. Below, heat maps show changes affecting a hub of 50 transcripts related to cell cycle and compromised in i Olfm2-null adipose tissues, as well as in our j human adipocyte cultures with impaired OLFM2 (si-OLFM2 and sh-OLFM2 cells vs controls). Heat maps represent z-scores based on gene-counts for each sample using the package Bioinfokit (2.0.8) for Python. Gene symbols inked in blue depict transcripts with FDR p < 0.05 in at least one fat depots and/or experimental approach in vitro. Venn diagrams summarize coincidences within genes significantly k down (31) and l up-regulated (12 transcripts) in Olfm2-null mice and human adipocytes with defective OLFM2 signal.
