Fig. 2: Correlation between the expression of TLR4⁺ILC2s and type 2 inflammations in polyp tissues.

A Representative contour plot analysis for TLR4⁺ILC2s (CD45⁺Lin^⁻CD127⁺CRTH2⁺CD161⁺TLR4⁺) in a single cell suspension of nasal polyp tissues from CRSwNP patients. B Representative histogram showing the expression levels of IL-17RB, IL-13, IL-5 and CD44 on TLR4⁺ILC2s and TLR4^⁻ILC2s in polyp tissues of CRSwNP patients. Shading (FMO) indicates isotype control. C Mean fluorescence intensity (MFI) for IL-17RB, IL-13, IL-5, and CD44 detected by flow cytometry on TLR4⁺ILC2s and TLR4^⁻ILC2s in CRSwNP patients (n = 15). Each symbol represents an individual patient, and bars represent mean ± SEM. P-values were calculated by two-tailed paired Wilcoxon tests. D, E Representative contour plot analysis for total ILC2s (CD45⁺Lin^⁻CD127⁺CRTH2⁺CD161⁺ cells) (D), TLR4⁺ILC2s and TLR4^⁻ILC2s (E) in nasal polyp tissues from CRSwNP patients. F Percentage of total ILC2s, TLR4⁺ILC2s and TLR4^⁻ILC2s in nasal polyp tissues of IL-5^⁻CRSwNP (n = 9) versus IL-5⁺CRSwNP patients (n = 29). Each symbol represents an individual patient, and bars represent mean ± SEM. P-values were calculated by two-tailed Mann-Whitney tests. G. Correlation between the frequency of TLR4⁺ILC2s and percentage of eosinophils, and concentrations of ECP, IL-13 and IL-5 in polyp tissues. Each symbol represents an individual patient. P and r-values were calculated by a two-tailed Spearman correlation test.