Fig. 3: Expression of TLR4⁺ILC2s in a mouse type 2 inflammatory model.

A. Experimental protocol. i.n., intranasal administration; HDM, house dust mite. B–E. Representative contour plot and quantification showing the percentage of total ILC2s (B), TLR4⁺ILC2s and TLR4^⁻ILC2s (C), IL-13⁺TLR4⁺ILC2s (D) and IL-13⁺TLR4^⁻ILC2s (E) in lung tissues of Rag1^⁻/⁻ mice on days 0, 17, 45, and 47 (n = 8 mice per group). Each symbol indicates one mouse, and bars represent mean ± SEM. Data were pooled from two independent experiments. P-values were calculated by two-tailed one-way ANOVA with Tukey’s Honestly Significant Difference test.