Fig. 6: Induction of type 2 airway inflammation by adoptive transfer of TLR4⁺ILC2s.

A Experimental protocol. i.n., intranasal administration; i.v., tail intravenous injection; HDM, house dust mite. B Representative contour plots analysis eosinophils in lung tissues of different groups. C–E Percentage of eosinophils in lung tissues (C), concentration of tissue IL-13 (D) and airway hyperreactivity (E) in Rag2^⁻/⁻Il2rg^⁻/⁻C57BL/6 J mice following transfer of ILC2 subsets from HDM-sensitized wild type C57BL/6 J mice (TLR4⁺ILC2 or TLR4^-ILC2 group, n = 4 mice per group; other group, n = 5 mice per group). F Representative image of MUC5AC immunohistochemistry staining picture (left) and statistics summarizing (right) in lung tissue of Rag2^⁻/⁻Il2rg^⁻/⁻C57BL/6 J mice (TLR4⁺ILC2 or TLR4^-ILC2 group, n = 4 mice per group; other group, n = 5 mice per group). Each dot indicates one mouse, and bars represent mean ± SEM. Results for (C–F) were confirmed in two independent experimental replicates, with representative data shown. P-values were calculated by two-tailed one-way ANOVA with Tukey’s Honestly Significant Difference test.