Fig. 8: Effect of recall challenge in vitro-generated trained ILC2s.
From: TLR4+group 2 innate lymphoid cells contribute to persistent type 2 immunity in airway diseases
A Schematic diagram for in vitro inducing trained ILC2s from sorted mouse TLR4⁻ILC2s. HDM, house dust mite; LPS, lipopolysaccharide. B, C Mean fluorescence intensity (MFI) of TLR4, IL-5, IL-13, Ki67 and IL-17RB in in vitro-generated trained ILC2s derived from TLR4⁻ILC2s. Data were pooled from three independent experiments. P-values were calculated by two-sided one-way ANOVA with Tukey’s Honestly Significant Difference test (B) or two-sided unpaired t test (C). D, E IL-5, IL-13 and Ki67 MFI in in vitro-generated trained ILC2s, which were pretreated with TAK242 (TLR4 antagonist, 1 h). Data were pooled from three independent experiments. P-values were calculated by a two-sided unpaired t test.
