Fig. 1: Neuropathic pain increases the levels of m⁶Am and PCIF1 protein in S1HL.

a m⁵C, ac⁴C, m⁶Am, m⁷G, and m⁶A levels in contralateral primary somatosensory cortex (S1HL) on days 7 and 21 after surgery (n = 8 mice). b Expression level of METTL3, METTL14, PCIF1, ALKBH5, and FTO protein in the contralateral S1HL after surgery (n = 10 mice). c, e Expression of PCIF1 protein in contralateral S1HL at different times after spared nerve injury (SNI; n = 10 mice) or chronic constriction injury (CCI; n = 8 mice) or sham surgery. d, f Quantitative analysis of Pcif1 mRNA expression in contralateral S1HL by qRT-PCR at different times post-SNI or post-CCI or sham surgery (n = 8 mice). g–k Expression of PCIF1 protein in contralateral S1HL in a model of cisplatin-induced neuropathic pain (Cisp), diabetic neuropathic pain (DNP) or chronic restraint stress (CRS) (n = 8 mice). i, j Expression of PCIF1 protein in contralateral S1HL at different times after complete Freund’s adjuvant (CFA)injection (Saline group: n = 8 mice, CFA group: n = 10 mice) or formalin (FM) injection (Saline group: n = 12 mice, FM group: n = 10 mice). l, m Co-expression analysis of PCIF1 with neuron (NeuN), astrocyte (S100β) or microglia (Iba1) immunofluorescence staining in S1HL of naïve mice (n = 4 mice). Scale bar, 200 μm. n Schematic showing isolation of GABAergic neurons and glutamatergic neurons from the L2/3, L5, and L6 layers of S1HL of mice subjected to SNI surgery for quantitative analysis of Pcif1 mRNA expression. o, p Expression of Pcif1 mRNA in GABAergic or glutamatergic neurons of S1 layers 2-6. n = 12 single cells form mice. The data are represented as mean ± SEM. For RNA modifications, mRNA and protein, S1 samples from two mice were pooled to create one sample. One-way ANOVA followed with Dunnett’s post hoc test was used for panels (a, b). Two-way ANOVA followed with Fisher’s LSD post hoc test was used for panels (c–f, i, j, o and p). Student’s unpaired t-test (two-tailed) was used for panels (g, h and k).