Fig. 3: Characterization of antifungal lipopeptaibiotic coniotins from the Coniochaeta fungus WAC11161.

a HR mass spectrum of compound 1 obtained with QToF mass spectrometry, showing the [M + H]⁺ ion at m/z 2057.2609. In-source fragmentation produced ions at m/z 506.36, 602.31, 690.48, 843.45, 931.62, 1126.65, and 1215.81. b MS/MS analysis of compound 1 using CID combined with a product ion scan (MS/MS) of nominal m/z 2057.26. The precursor ion is indicated with a blue square. The structure of the lipopeptide 1 (termed coniotin A) is displayed above, along with its collision-induced fragmentation pattern, which corresponds to the detected b ions in the MS/MS spectrum. The b ions are labeled in blue, and the y ions are labeled in red. c The structures of antifungal lipopeptaibiotics analogs 1, 2, 3, and 4, identified from the Coniochaeta fungus WAC11161, termed coniotin A, B, C, and D. d HR mass spectrum of coniotin B obtained with QToF mass spectrometry, showing the [M + H]⁺ ion at m/z 2056.2781 (calculated for C₉₈H₁₇₁N₂₂O₂₅, 2056.2780). e HR mass spectrum of coniotin C obtained with QToF mass spectrometry, showing the [M + H]⁺ ion at m/z 2043.2464 (calculated for C₉₇H₁₆₈N₂₁O₂₆⁺, 2043.2464) and the [M+Na]⁺ ion at m/z 2065.2255. f HR mass spectrum of coniotin D obtained using QToF mass spectrometry, showing the [M + H]⁺ ion at m/z 2042.2689 (calculated for C₉₇H₁₆₉N₂₂O₂₅⁺, 2042.2624), the [M+Na]⁺ ion at m/z 2064.2536, and the [M + K]⁺ ion at m/z 2080.2316.