Fig. 6: Metabolic flux map upon overexpression of LplA in CHO cells. | Nature Communications

Fig. 6: Metabolic flux map upon overexpression of LplA in CHO cells.

From: Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein

Fig. 6

Extracellular fluxes of glycolysis-related metabolites (a) and amino acids (b) into cells after 48 h of adding [U-13C] glucose. Asterisk symbol represents the metabolite is depleted after 24 h. Positive values represent the consumption fluxes, while negative values represent the production flux. Heatmap of isotopic labeling of intracellular metabolites (c) and MID data (d–r) after 24 and 48 h of adding [U-13C] glucose. s Metabolic flux distribution of engineered cell lines. Data were represented as mean ± SD (n = 3, n biologically independent samples). Multiple unpaired two-sided t-test was used for the statistical analyses for (a, b). P adjustments were made for multiple comparisons. G6P glucose-6-phosphate, 3PG 3-phosphoglycerate, Cit citrate, AKG alpha-ketoglutarate, Suc succinate, Fum fumarate, Mal malate, OAA oxaloacetate, Glu glutamate, Gln glutamine, Asp aspartate, Asn asparagine, Ser serine, Gly glycine, Lac lactate, Ala alanine. Source data are provided as a Source Data file.

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