Fig. 1: Map-based cloning of the candidate gene for the tin7 mutation.
From: The TaNHLP1-TaRACK1A module regulates tillering via abscisic acid signaling in wheat
a Morphology of WT (G1812) and the tin7 mutant grown in the greenhouse for 30 days. Scale bar = 5 cm. Tiller number comparison b and plant morphology c between WT and the tin7 mutant at the filling stage. Data in b are presented as mean ± SD (n = 15 individual plants) and were analyzed using Student’s t test (two-sided). d Tiller buds from WT and the tin7 mutant are shown, with white arrows indicating the positions of the first primary tiller. Scale bar = 10 mm. e Fine mapping using F2 and F3 recombinants narrowed down the candidate gene to a 387 kb interval between markers IN13 and IN5. The numbers below the markers indicate the number of recombinants. Genes annotated within the IN13-IN5 interval of the T. urartu v2.0 reference genome. The red arrow indicates TuNHLP1. f Gene structure and sequence variation of the TuNHLP1 gene. Black boxes represent exons, and black horizontal lines represent introns. Nucleotide change and mutation position between WT (black) and the tin7 mutant (red) are highlighted, with the coordinates starting from the initial ATG of the open reading frame. g Schematic diagram of the predicted TuNHLP1 protein structure. SP, signal peptide; NHL, NHL (NCL-1, HT2A, and LIN-41) repeat domain; TM, transmembrane helix region. Amino acid change and mutation position between WT (black) and the tin7 mutant (red) are highlighted, with the coordinates starting from the initial Met. Source data are provided as a Source Data file.
