Fig. 1: Generation of conditionally immortalized hematopoietic progenitor cell lines based on Hoxa7 and TPO.
From: Murine hematopoietic progenitor cell lines with erythroid and megakaryocyte potential
a Growth characteristics of Hoxa7-TPO cells. BM cells from H2K GFP transgenic mice were transduced with an estrogen-regulated form of Hoxa7 (ERHBD-Hoxa7) or a vector control and cultured in the presence of estrogen and TPO. Numbers of live cells were determined at the time points indicated. Source data are provided as a Source Data file. b Phase contrast microscopy of stably growing Hoxa7-TPO cells. c Cytology analysis of Hoxa7-TPO cells based on May-Grünwald-Giemsa staining. d Cell survival analysis of Hoxa7 cells in the absence of TPO. TPO was withdrawn at day 0 and the number of live cells was determined during time. Source data are provided as a Source Data file. Scale bars (B, C, E) = 20 μm. Error bars represent standard deviation of five biological replicates. e Flow cytometry analysis of Hoxa7-TPO cells. Dark grey, indicated antibodies; light grey, isotype controls.
