Fig. 4: In vivo potential of Hoxa7-TPO cells.

a Flow cytometry analysis of the PB of mice that were adoptively transferred with GFP-expressing Hoxa7-TPO cells for indicated time periods. b Quantitative analysis of Hoxa7-TPO-derived platelets and RBC in the PB of mice adoptively transferred with independently established Hoxa7-TPO cell populations (n = 6) for indicated time periods. Symbols represent independently established cell populations. Source data are provided as a Source Data file. c, d Immuno-fluorescence analysis of washed Hoxa7-TPO-derived and endogenous platelets that were seeded on chamber slides coated with Poly-L-lysine (c) or fibrinogen (d), incubated with antibodies against indicated proteins or phalloidin, followed by confocal microscopy. Scale bars = 10 μm. e Flow cytometry-based gating strategy (left) and microscopy images (right) of May-Grünwald-stained cytospins of FACS-purified cell populations 14 days after adoptive transfer of Hoxa7-TPO cells. I = BM-derived RBC; II = Hoxa7-TPO-derived Ter119⁺ RBC; III = Hoxa7-TPO-derived Ter119^- RBC. Scale bars = 10 μm. f Flow cytometry-based quantification of Hoxa7-TPO- and Hoxb8-FL-derived white blood cells (WBC) in the PB 7 days after adoptive transfer. Data represent mean ± SD for 5 (Hoxa7-TPO) and 3 (Hoxb8-FL) adoptively transferred mice. ****P < 0.0001. Two-tailed unpaired t test. Source data are provided as a Source Data file. g, h Quantitative analysis of platelets (g) and RBC (h) in the PB of mice that were adoptively transferred with 15 clones derived from Hoxa7-TPO cells. Each symbol represents data obtained from individual mice transferred with one individual clone. Source data are provided as a Source Data file.