Fig. 5: LifeAct signal and mean curvature of the CL of the ventricle of the zebrafish heart.
From: Capturing nematic order on tissue surfaces of arbitrary geometry
a, b 3D reconstruction of the ventricle at 72 and 120 hpf. The signal within a distance of 3 μm to the surface is shown. Bright areas of the heart show the fluorescence signal of F-actin (-0.2myl7:Lifeact-mNeongreen). The ventricle is superimposed with nematic directors with a distance of about 10 μm, determined based on the membrane signal shown in (d, e), whose color code corresponds to the mean intensity signal in respect to the background, see also Supplementary Movie 4 and Supplementary Movie 5. Region I and II (red dots, encircled in red) were used for quantification and comparisons in (c). d, e 3D reconstruction of the ventricle at 72 and 120 hpf. Dark areas of the heart indicate the fluorescence signal of the membrane (myl7:BFP-CAAX). The color code of the ventricle is superimposed with nematic directors, whose color code corresponds to the mean curvature, see also Supplementary Movie 6 and Supplementary Movie 7. Region I and II (white dots, encircled in white) were used for quantification and comparisons in (f). c, f Each box plot contains data points of these regions of zebrafish hearts at 72 and 120 hpf, five analyzed hearts each, NI,72hpf = 66, NII,72hpf = 59, NI,120hpf = 62, NII,120hpf = 63. The black dots are the mean values of selected data points per analyzed heart. Each box shows the median (red line), 25th and 75th percentiles (box), maximum and minimum without outliers (whiskers), and 95% confidence interval of the median (notches). P-values were calculated from Dunn’s test of multiple comparisons after a significant Kruskal-Wallis test.
