Table 5 Summary of assay specifics for kinase subpanel
Enzyme | Vendor | Cat No. | Enzyme conc. (nM) | ATP conc. (μM) | Substrate | Reaction time (min) |
|---|---|---|---|---|---|---|
MAP4K2 | Carna | 07-111 | 1.40 | 100 | FL-peptide 25 (PerkinElmer, cat. 760388) | 90 |
FLT3 | Carna | 08-154 | 0.63 | 100 | FL-peptide 2 (PerkinElmer, cat. 760346) | 90 |
HIPK2 | Carna | 04-136 | 3.49 | 10 | FL-peptide 24 (PerkinElmer, cat. 760387) | 90 |
TRKA | Carna | 08-186 | 0.90 | 100 | FL-peptide 27 (PerkinElmer, cat. 760424) | 90 |
CLK4 | Eurofins | 14-917 | 2.50 | 100 | FL-peptide 34 (PerkinElmer, cat. 760643) | 120 |
DYRK1a | Carna | 04-130 | 2.00 | 20 | FL-peptide 24 (PerkinElmer, cat. 760387) | 90 |
CDK9 | Carna | 04-110 | 4.30 | 10 | FL-peptide 34 (PerkinElmer, cat. 760643) | 90 |
SLK | Carna | 07-129 | 0.07 | 50 | FL-peptide 33 (PerkinElmer, cat. 760642) | 90 |
AXL | Carna | 08-107 | 1.67 | 50 | FL-peptide 30 (PerkinElmer, cat. 760430) | 90 |
JAK2 | Carna | 08-045 | 0.70 | 20 | FL-peptide 22 (PerkinElmer, cat. 760366) | 90 |
NEK2 | Carna | 05-226 | 4.70 | 100 | FL-peptide 11 (PerkinElmer, cat. 760355) | 90 |
AKT1 | Carna | 01-101 | 0.25 | 30 | FL-peptide 6 (PerkinElmer, cat. 760350) | 90 |
ABL | Carna | 08-001 | 1.00 | 20 | FL-peptide 2 (PerkinElmer, cat. 760346) | 90 |
KIT | Carna | 08-156 | 3.66 | 400 | FL-peptide 30 (PerkinElmer, cat. 760430) | 90 |
PDGFRβ | Carna | 08-158 | 1.50 | 30 | FL-peptide 30 (PerkinElmer, cat. 760430) | 90 |
Ret | Carna | 08-159 | 0.60 | 10 | FL-peptide 22 (PerkinElmer, cat. 760366) | 90 |
IRAK1 | Carna | 09-101 | 5.00 | 30 | FL-peptide 10 (PerkinElmer, cat. 760354) | 180 |
MERTK | Carna | 08-108 | 0.20 | 50 | FL-peptide 27 (PerkinElmer, cat. 760424) | 90 |
CSNK1E | Carna | 03-104 | 10.00 | 20 | FL-peptide 16 (PerkinElmer, cat. 760360) | 90 |
ERK2 | Carna | 04-143 | 3.33 | 50 | FL-peptide 8 (PerkinElmer, cat. 760352) | 90 |
