Fig. 1: CELF1 expression in iWAT is upregulated upon thermogenic activation.

a Venn analysis of overlapped genes between those significantly upregulated in response to cold stimulation from GSE148361 and GSE110420 (p < 0.01) and canonical RNA-binding proteins (RBPs) annotated in the RBP database. b Celf1 and Ucp1 expression in iWAT from wild-type (WT) mice exposed at 4 °C or RT for 4 days by microarray analysis from GSE148361 (n = 4 mice in each group). Celf1 (c) and Ucp1 expression (d) in iWAT from WT mice exposed at 5 °C (n = 6) or thermoneutrality (28 °C; n = 5) for 10 days by microarray analysis from GSE110420. e, f WT male mice housed at RT or 4 °C for 24 h. e qPCR analysis of Celf1 and Ucp1 mRNA levels in iWAT (n = 12 mice for RT group, n = 11 mice for 4 °C 24 h group). f Immunoblot analysis of the indicated protein in iWAT of mice (n = 4). g, h WT mice were injected with CL316,243 intraperitoneally twice, once per 12 h. g qPCR analysis of Celf1 and Ucp1 mRNA levels in iWAT (n = 7 biological replicates). h Immunoblot analysis of the indicated protein in iWAT (n = 4  biological replicates). i, j WT mice were housed at RT or 4 °C for 24 h or 7 days. i qPCR analysis of Celf1 and Ucp1 mRNA levels in iWAT of mice (n = 3 for RT and 4 °C 24 h group, n = 4 for 4 °C 7 d group). j Immunoblot analysis of the indicated protein in iWAT of mice (n = 3). Data are presented as mean ± SEM, statistical significance was determined by two-tailed unpaired Student’s t-test (b–e, g) or one-way ANOVA (i). Source data is provided as a Source Data file.