Fig. 2: Adipocyte Celf1 deficiency suppresses cold-induced iWAT beiging and energy expenditure.
From: Adipocyte RNA-binding protein CELF1 promotes beiging of white fat through stabilizing Dio2 mRNA
a–j 8-week-old Celf1fl/fl and Celf1AKO mice were housed at 4 °C for 6 h. a qPCR analysis of the mRNA level of thermogenic genes in iWAT (n = 10 biological replicates). b Immunoblot analysis of the indicated protein in iWAT (n = 3 biological replicates). c Representative images of H&E staining and UCP1 immunohistochemistry (IHC) staining of iWAT, scale bar, 50 μm. VO2 (d–e) and heat production (n = 6 mice for 22 °C group; n = 7 mice group for 4 °C) (f) of Celf1fl/fl and Celf1AKO mice at 22 °C and 4 °C during time (left), and average values (right) (n = 6 mice for 22 °C; n = 7 mice for 4 °C). g Rectal temperatures of Celf1fl/fl (n = 6) and Celf1AKO (n = 5) mice at different time points during acute cold stimulation for 6 h. Heat maps analysis of differentially expressed genes (h), Gene Ontology (GO) (i), and Kyoto Encyclopedia of Genes and Genomes (KEGG) (j) analysis of downregulated genes in iWAT of Celf1AKO mice according to RNA-seq results. Data are presented as mean ± SEM, statistical significance was determined by two-tailed unpaired Student’s t-test (a, e, f, g). Source data is provided as a Source Data file.
