Fig. 3: Adipocyte CELF1 augments isoproterenol-induced activation of beige adipocytes and mitochondrial respiration in vitro.

a–d Primary preadipocytes isolated from iWAT of WT mice were differentiated into adipocytes. qPCR (n = 6 for PBS or ISO group; n = 3 for DMSO or FSK group) or immunoblot analysis (n = 3) of CELF1 and UCP1 expression in primary adipocytes treated with PBS or ISO (a, b) and DMSO or FSK (c, d). e–k Primary adipocytes were infected with control shLacZ or shCelf1 adenovirus, control Vector or Celf1-overexpressing (Celf1) adenovirus, and then treated with PBS or ISO for 10 h before harvesting. e qPCR analysis of the mRNA level of thermogenic genes in primary adipocytes infected with shLacZ or shCelf1 adenovirus (n = 4 biological replicates). f Immunoblot analysis of the indicated protein in primary adipocytes infected with shLacZ or shCelf1 adenovirus (n = 3 biological replicates). g qPCR analysis of the mRNA level of thermogenic genes in primary adipocytes infected with Vector or Celf1-overexpressing adenovirus (n = 4 biological replicates). h Immunoblot analysis of the indicated protein in primary adipocytes infected with Vector or Celf1-overexpressing adenovirus (n = 3). i Representative analysis of OCRs of primary adipocytes infected with shLacZ or shCelf1 adenovirus in the left panel; The statistical result of basal respiration is shown in the right panel (n = 4 biological replicates). j Statistical results of maximum respiration and proton leakage respiration of panels (n = 4 biological replicates). k Representative analysis of OCRs of primary adipocytes infected with Vector or Celf1-overexpressing adenovirus in the left panel; The statistical results of basal respiration, maximum respiration, and proton leakage respiration are shown in the right panel (n = 4 biological replicates). Data are presented as mean ± SEM; statistical significance was determined by two-tailed unpaired Student’s t-test (a, c, e, g, i–k). Source data is provided as a Source Data file.