Fig. 4: CELF1 binds to the 3′UTR of mRNA and targets to thyroid hormone signaling pathway.
From: Adipocyte RNA-binding protein CELF1 promotes beiging of white fat through stabilizing Dio2 mRNA
a Immunoblot analysis of CELF1 protein immunoprecipitated by CELF1 antibody; 10% Immunoprecipitation (IP) cell lysate was used as the input. RNA Immunoprecipitation (RIP) was conducted with differentiated primary adipocytes treated with ISO for 10 h. (Images are representative of three independent experiments) b Gene location distribution of CELF1 target according to the peak density of the RIP-Seq analysis. c Enriched sequences from the CELF1 RIP-Seq data defined by the Homer software (p < 0.05). d GO biological process analysis of CELF1 targets. e KEGG pathway enrichment analysis of CELF1 targets. f Venn analysis of overlap between differentially expressed genes from RNA-seq and enriched genes from RIP-seq. g KEGG pathway enrichment analysis of overlap genes between differentially expressed genes from RNA-seq and enriched genes from RIP-seq. h RIP-qPCR was conducted to verify target genes enriched in the thyroid hormone signaling pathway (n = 3 biological replicates). Data are presented as mean ± SEM; statistical significance was determined by two-tailed unpaired Student’s t-test (h). Source data is provided as a Source Data file.
