Fig. 2: Constitutive AnkB loss induces mild, muscle-type-specific changes in sarcolemma dystrophin. | Nature Communications

Fig. 2: Constitutive AnkB loss induces mild, muscle-type-specific changes in sarcolemma dystrophin.

From: Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity

Fig. 2

a Volcano plot of differentially expressed genes in GC from SKM-AnkB-KO mice (n = 6 mice/genotype). Blue and red dots, respectively, denote downregulated and upregulated transcripts. Western blots and quantification of total dystrophin levels in GC (b, c) and SOL (d, e) normalized to total protein stain, and graphed as fold-change over WT levels (n = 4 mice/genotype). Representative images of GC (f) and SOL (k) from 4-mo mice. Asterisks denote fibers lacking dystrophin. Arrowheads indicate centrally located nuclei (CLN). Scale bar, 200 µm. Percent of dystrophin-negative fibers (g, l), dystrophin membrane levels relative to WT (h, m), fiber size distribution (i, n), and percent of fibers with CLN (j, o), all quantified from GC (f-j) and SOL (k–o) sections from 4-mo male mice (n = 3 mice/genotype). Error bars are mean ± S.D. Statistical differences were determined by a two-tailed unpaired t-test with a significance threshold of p < 0.05.

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