Fig. 2: The spatial occupancy and dynamics of CCT tails in apo TRiC.
From: Visualizing dynamic tubulin folding in chaperonin TRiC from nonnative nucleus to final native state
A The sliced side view of apo TRiC reconstruction in dark gray color without B factor sharpening. B The complete modeling of CCT tails. The left figure shows the CCT tails in a single TRiC ring of the bottom view, with the location of the first N tail residue (orange circle) and the last C tail residue (purple diamond). The resolved CCT tails are highlighted in thicker lines. The right figure shows the transparent side view of TRiC with CCT C tails in orange and N tails in green. C The workflow of CryoDRGN training and analysis of heterogeneity in apo TRiC particles. D The sliced side view of TRiC volume reconstructed from latent space embedding shows the CCT tails density in orange color indicating intra-ring termini interaction. E The sliced side view of TRiC volume reconstructed from latent space embedding shows the CCT tails density at the inter-ring interface, indicating inter-ring tails interaction. F The single TRiC ring model from the bottom view displaying the electrostatic distribution on the TRiC chamber wall. Blue color indicates positively charged distribution while red color indicates negatively charged distribution. G The single TRiC ring model from the bottom view displaying the electrostatic distribution on the CCT tails. Blue color indicates positively charged distribution while red color indicates negatively charged distribution. H The segregation of CCT tails into a positively charged cluster and a negatively charged cluster across the two rings of TRiC. I The schematic shows positively charged C tails (CCTs 3,6,8,7) adjacent to positively charged wall (CCTs 1,3,6,8), and negatively charged C tails (CCTs 5,2,4,1) adjacent to negatively charged wall (CCTs 7,5,2,4).
