Fig. 4: Exogenous JAG1-peptide restores growth and PI3K-Akt-mTOR signaling to near normal levels in Jag1-deficient cochlear organoids.
From: The Notch ligand Jagged1 plays a dual role in cochlear hair cell regeneration
a Experimental scheme. Cochlear epithelial cells from stage P2 mice were used to establish control and Jag1 cKO organoid cultures, which were analyzed on day 10 of expansion. Organoids were treated with JAG1-Fc peptide on day 3 of expansion and cultured for seven additional days. b BF images of control and Jag1 cKO organoids treated with or without JAG1-Fc peptide on day 10 of expansion. Scale bars = 400 µm. c, d Quantification of organoid forming efficiency (c) and organoid diameters (d) for (b) (n = 4 animals per group, two independent experiments). e Immunoblots of p-Akt, pS6, p-4EBP1, JAG1, and β-actin protein in control and JAG1 peptide-treated organoids on day 10 of expansion (three independent experiments). Graphed are individual data points and mean ± standard deviation. One-way ANOVA with Tukey’s correction was used to calculate exact p-values. Source data are provided as a Source Data file.
