Fig. 1: OGT inhibits IAV infection via both catalytic activity-dependent and -independent manner.
From: O-GlcNAc transferase plays dual antiviral roles by integrating innate immunity and lipid metabolism
a–g Mouse embryonic fibroblasts (MEFs) generated from Ogtfl/fl, Ogt-K908Afl/fl, Ogt KO and OgtK908A mice were infected with PR8 or PR8-GFP virus at a multiplicity of infection (MOI) = 1 for 12 h. Viral mRNAs (a), viral titers (b), expression of IAV HA and NA proteins (c), and virus infected cells (d) were measured by RT-PCR, TCID50 assays, immunoblotting with corresponding antibodies and flow cytometry respectively. e Gene transcripts including Ifna4, Ifnb1, Mx1, Isg15, Cxcl10, Il6, and Tnfa in the cells were measured with RT-PCR. f Immunoblotting of phosphorylated TBK1, IRF3, NF-κB signaling molecules and protein O-GlcNAcylation was performed with indicated antibodies. g Immunoprecipitated MAVS from the MEFs was assessed for O-GlcNAcylation with anti-O-GlcNAc antibody. Data are representative of three independent experiments (a, b and d, e). Statistical significance was determined by two-way ANOVA followed by Tukey’s test (a, b and d, e), P values of statistical comparisons are shown in each graph. The error bars represent SEM from n = 3 independent biological replicates (a, b and d, e). Source data are provided as a Source Data file.
