Fig. 2: Selection of combinatorial antibodies targeting the fusion core of HR1. | Nature Communications

Fig. 2: Selection of combinatorial antibodies targeting the fusion core of HR1.

From: A broadly neutralizing antibody recognizes a unique epitope with a signature motif common across coronaviruses

Fig. 2

a Enrichement of combinatorial antibodies targeting HR1FCSC2. The input and output phage numbers in the three rounds of panning are summarized in the table (mean value) and histogram. Data (mean ± SD) are calculated from three plates with different dilution gradients. b Inferred germline genes encoding the variable regions of the enriched antibodies and their corresponding mutation frequencies. c Cross-interaction of 3D1 with the HR1FC peptides of SARS-CoV-1 and SARS-CoV-2, along with 3D1 epitope mapping against the full-length HR1 fusion core (HR1FC), the C-terminal of HR1FC (HR1C), the N-terminal of HR1FC (HR1N), and the minimal pepDVVNQN. d Cross-neutralization of 3D1 against the SARS-CoV-1 (green) and SARS-CoV-2 (blue) pseudoviruses. Each titration point in (c, d) represents the mean of independent replicates, with standard deviations shown as error bars (mean ± SD, n = 3).

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