Fig. 3: Panx1 knockout mitigates renal senescence in vivo. | Nature Communications

Fig. 3: Panx1 knockout mitigates renal senescence in vivo.

From: Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury

Fig. 3

a Representative SA-β-gal staining in renal tissue. Scale bars, 50 µm. b Representative immunofluorescent images of C12FDG (green), Panx1 (red), and DAPI (blue) in kidneys. Scale bars, 40 µm. c qPCR analysis of senescence markers (Cdkn2a, Cdkn1a, Tp53). d Relative mRNA levels of SASP factors (IL-1β, IL-6, TGF-β). For all panels, images are representative, and data are from WT Sham (n = 6), K/O Sham (n = 6), WT d14-IRI (n = 8), K/O d14-IRI (n = 7), WT d28-IRI (n = 8), K/O d28-IRI (n = 8) mice. The qPCR results are shown as the fold change compared with the WT-sham group. All quantitative data are presented as means ± SD. P values were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Exact P values are provided in the Source Data file. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page