Fig. 8: Panx1 downregulation mitigates mitochondrial dysfunction. | Nature Communications

Fig. 8: Panx1 downregulation mitigates mitochondrial dysfunction.

From: Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury

Fig. 8

a, b Mitochondrial function in irradiated HK-2 cells after Panx1 knockdown. a Representative images and quantification of mitochondrial membrane potential using JC-1 staining. b Representative images and quantification of mitochondrial ROS using MitoSOX staining. For a, b images are representative of n = 3 independent biological replicates. Scale bars, 30 µm. c, d Mitochondrial analysis in renal tissue from wild-type (WT) and Panx1 knockout (K/O) mice post-uIRI. c Representative TEM images and quantification of mitochondrial vacuolization. Swollen or vacuolated mitochondria are indicated with red asterisks. Data are from n = 3 fields per group, with each group containing 20–30 mitochondria. Scale bars, 1 µm. d Representative images and quantification of mitochondrial ROS (MitoSOX). Images are representative of WT Sham (n = 6), K/O Sham (n = 6), WT d14-IRI (n = 8), K/O d14-IRI (n = 7), WT d28-IRI (n = 8), K/O d28-IRI (n = 8) mice. Scale bars, 50 µm. Data are presented as means ± SD. P values were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Exact P values are provided in the Source Data file. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page