Fig. 1: The framework of in vivo spatial-isotope deep tracing.
From: Spatial isotope deep tracing deciphers inter-tissue metabolic crosstalk
a Overview of the spatial isotope deep tracing framework using LC‒MS/MS and AFADESI-MSI. Created in BioRender. Li, X. (https://BioRender.com/sakosq5). b High-coverage identification of labeled metabolites in the mass range (m/z 70−1200) under three LC conditions following U-13C glucose infusion. c Numbers of labeled metabolite ions and isotopologues after U-13C glucose and U-13C glutamine infusion. The isotopologues counted refer to those with labeling fractions greater than zero across all three samples. d Labeling profile of the metabolite ion m/z 385.2226 across serum and organs. e Classification of the labeled metabolites in various matrices following infusion of U-13C glucose (left) and U-13C glutamine (right). Metabolite ions detected under different LC‒MS conditions were counted once. f The distributions of shared and unique labeled metabolites across the body. Each column represents the number of labeled metabolites identified in the specific matrix, which are displayed as black solid dots in the lower section of the plot. Source data are provided as a Source Data file.
