Fig. 2: MSITracer enables spatial isotope tracing from whole-body animals to microregions of tissues.
From: Spatial isotope deep tracing deciphers inter-tissue metabolic crosstalk
a Schematic diagram of the MSITracer workflow. b AFADESI-MS images of native glutamate and 13C-enriched isotopologues in whole-body mice after two 13C-nutrient infusions. The organs of interest are delineated with dashed lines in different colors. Intensity shown as relative abundance (normalized to 100%). c, d AFADESI-MS images of native glutamate and representative 13C-isotopologues in the kidney (c) and brain (d). For each figure, from left to right, the images correspond to the unlabeled, U-13C glucose-infused, and U-13C glutamine-infused samples. Statistics were calculated using one-way ANOVA and post hoc Tukey’s correction, based on three replicates, each with two representative regions of interest (ROI) selected from each slice (mean ± SD). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Adjusted p values for comparisons reaching statistical significance are provided in Supplementary Table 2. Signal intensity is shown in ion counts. Oc, outer cortex; Ic, inner cortex; Me, medulla; Pe, pelvis; AM, amygdala; HY, hypothalamus; TH, thalamus; IC, internal capsule; HP, hippocampus; CC, corpus callosum; CTX, cerebral cortex. Source data are provided as a Source Data file.
