Fig. 5: Comparison of the bound type-2 Arg/N-degrons. | Nature Communications

Fig. 5: Comparison of the bound type-2 Arg/N-degrons.

From: Structural basis for the recognition and ubiquitylation of type-2 N-degron substrate by PRT1 plant N-recognin

Fig. 5

a Superposition of ZZ domain complexes with peptides, YKFG (colored cyan for protein and white for peptide), FKFG (magenta for protein and slate for peptide), and WAAG (green for protein and salmon for peptide). b Close-up view of the binding mode of the ZZ domain with Nt Tyr, Phe, and Trp residues. Type-2 bulky hydrophobic N-degrons are positioned near the key residues of the ZZ domain. The hydrophobic network is highlighted as a transparent brownish oval. The colors are consistent with those in (a). c Isothermal calorimetry results of the FL PRT1WT with type-2 bulky hydrophobic Arg/N-degrons. KD values: YAA = 23.3 ± 0.958 μM; FAA = 5.45 ± 0.212 μM; WAA = 2.85 ± 0.164 μM. The detailed parameters are listed in Supplementary Table 3.

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