Fig. 2: Loss of function of MRE11 affects the chromatin binding of other DSB end resection factors, RAD52 and POLD3 following replication stress in U2OS cells. | Nature Communications

Fig. 2: Loss of function of MRE11 affects the chromatin binding of other DSB end resection factors, RAD52 and POLD3 following replication stress in U2OS cells.

From: DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells

Fig. 2

a Experimental workflow of cell synchronization for analyzing the chromatin-bound proteins at different stages of the cell cycle following siRNA to deplete MRE11 and APH treatment in S-phase. b Western blot analysis of soluble or chromatin-bound fraction of BRCA1, WRN, RAD50, BLM, DNA2, CtIP, phosphorylated CtIP, NBS1, POLD3, RAD52, RAD51, and RPA32 at the indicated cell cycle phases. A black arrow indicates the band of BLM detected with BLM antibody, while the star indicates a non-specific band. GAPDH or Histone H3 was used as loading control for soluble and chromatin bound proteins respectively. This experiment was repeated independently with similar results for three times. Source data are provided as a Source Data file.

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