Fig. 6: CdtB activates SREBP1 via mitochondria-mediated mTOR/P70S6K signaling pathway and the interaction with NONO. | Nature Communications

Fig. 6: CdtB activates SREBP1 via mitochondria-mediated mTOR/P70S6K signaling pathway and the interaction with NONO.

From: Helicobacter hepaticus promotes hepatic steatosis through CdtB-induced mitochondrial stress and lipid metabolism reprogramming

Fig. 6

a, b Immunofluorescence images of SREBP1 in mouse liver organoids infected with wild type (WT) or CdtB knockout (ΔCdtB) H. hepaticus (a) or treated with CdtA/C or holotoxin CDT (b) for 48 h. c Co-immunoprecipitation assay demonstrating the interaction between CdtB and NONO in Hep3B cells. Cells were transfected with either pCMV-Myc-EGFP or pCMV-Myc-EGFP-CdtB for 24 h prior to immunoprecipitation. d Immunohistochemical detection of γH2AX in liver sections from mice administered rAd-vector or rAd-CdtB at 17, 42, 60 days post-infection (DPI). e Western blot analysis of NONO expression in Hep3B cells following infection with WT or ΔCdtB H. hepaticus. f Immunofluorescence images of SREBP1 in Hep3B cells with either NONO overexpression or NONO knockout, transfected with pCMV or pCMV-CdtB. g Western blot analysis of SREBP1 in nuclear fractions of Hep3B cells and Hep3B NONO-knockout cells transfected with pCMV-Myc-EGFP or pCMV-Myc-EGFP-CdtB for 24 h. Cytoplasmic (cyto) and nuclear components (nuc) were probed with β-actin and CTCF antibodies, respectively. Scale bars: 20 µm (a, b), 50 µm (f) or 100 µm (d). Source data are provided as a Source Data file.

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