Fig. 3: Immune cell subclustering identified subsets of senescent-like cells. | Nature Communications

Fig. 3: Immune cell subclustering identified subsets of senescent-like cells.

From: Spatially-restricted inflammation-induced senescent-like glia in multiple sclerosis and patient-derived organoids

Fig. 3

a Immune cell subset based on snRNAseq. b Immune cell subclustering (UMAP plots) and annotation based on Absinta et al.8 Heatmap displaying gene module scores between subclusters and Absinta et al.8. c Percentage of the different immune cell subclustering by location and pathological stage. d Heatmap showing the expression of genes included in the SenMayo signature in microglia by tissue location and pathological stage. e Quantification (%) of SenMayo-defined senescent cells in each immune subclustered population demonstrating the presence of subsets of senescent-like cells in each subcluster. f Representative multiplex immunostaining showing the co-existence of p16INK4a positive and negative IBA1+, MHCII+, and CD68+ microglia at the chronic active lesion edge. g Correlation between the SenMayo signature score and the GO negative regulation of autophagy signature score by cell type (n = 43 samples, Pearson correlation coefficient r = 0.48, p < 0.0001, equation y = 0.3555*x + 0.0747). Each dot in the scatter plot represents the average of the scores per sample, cell type, and signature. h LFB-PAS staining showing lipofuscin-laden microglia cells at the chronic active lesion edge (arrows, magnified views). DC dendritic cells, MG microglia, MIMS microglia inflamed in MS, CAL chronic active lesion, PV M0 perivascular macrophages, WM white matter, CX cortex, LFB-PAS Luxol fast blue-periodic acid Schiff.

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