Fig. 7: Senescent-like microglial cells-driven rewiring of cellular communication in MS tissue. | Nature Communications

Fig. 7: Senescent-like microglial cells-driven rewiring of cellular communication in MS tissue.

From: Spatially-restricted inflammation-induced senescent-like glia in multiple sclerosis and patient-derived organoids

Fig. 7

a Workflow schematic of the cell-cell communication analysis using both snRNAseq and spatial transcriptomic datasets (CellChat, NICHES tools). Created in BioRender. Absinta, M. (2025) https://BioRender.com/d0hxq2g. b Circle plot representing the comparison between the cellular communication (ligand-receptors [LR] pairs) between senescent-like microglia and other cell populations vs. non-senescent microglia and other cell populations using CellChat on snRNAseq data. Red lines indicate increased number of interaction ligand-receptors pairs, while blue line reduced number of interactions. c Chord diagram showing detailed communication through upregulated pathways and providing insights into the autocrine- vs. paracrine-acting pathways for senescent microglia using CellChat. Significant upregulation was defined by an interaction with p < 0.05 and logFC for ligand expression >0.2. Colours correspond to the different cell-types. d Spatially mapping of prioritized ligand-receptor pairs involving senescent-like microglia (SPP1-CD44, TREM2-APOE, HLADB1-CD4, NAMPT-ITGAV5, PSAP-GPR37) within the Visium spot and their relative quantification (mean ± SD) by location and pathological stage (n = 7 locations/pathological stages from 12 Visium slides, ANOVA p < 0.0001 for all pairs, except PSAP-GPR37, post-hoc multiple comparison analysis *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). Representative icons with ligand-receptors pairs created in BioRender. Absinta, M. (2025) https://BioRender.com/d0hxq2g.

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