Fig. 1: Whole-brain 3D reconstruction and visualization of long-range monosynaptic inputs to histaminergic neurons.

a Experimental scheme and timeline for labeling long-range monosynaptic inputs to histaminergic neurons in mouse brain. rAAV-EF1α-DIO-mCherry-F2A-TVA-WPRE-hGH polyA, rAAV-EF1α-DIO-oRVG-WPRE-hGH polyA, and RV-ENVA-ΔG-EGFP was injected into TMN of HDC-CreERT2 adult mice. b Representative images of TVA-mcherry and RV-eGFP expression on TMN. This experiment was repeated independently with similar results for three times. c Representative images of coronal sections with several distance from bregma for RV-eGFP expression of mouse brains, with viruses injected into TMN bilaterally. d Drawings of the distribution of starter cells from several coronal sections containing TMN of sample mice. n = 3. e Main steps of data generation, acquisition, and processing in fMOST imaging. Raw data were analyzed by NeuroGPS system and manual correction. f Fluorescent-labeled information visualization of registered RV-eGFP-expression neurons of mouse brain (Top: horizontal and full view; Bottom: coronal and sagittal views). g Fluorescent-labeled neuron proportions of main upstream brain regions of histaminergic neurons from 5 mice. See the details and list of abbreviations in Supplementary Tables 1 and 2. All data are presented as mean ± SEM.