Fig. 2: Layer distribution and co-projection characterization of upstream cortical neurons that target histaminergic neurons.

a Fluorescent-labeled information visualization of registered RV-eGFP-expression cortical neurons of mouse brain (Top: coronal and sagittal views; Bottom: horizontal view and coronal sketch map). b Fluorescent-labeled neuron proportions of all cortical brain regions of histaminergic neurons. n = 5 (derived from different mice). c Representative image of the specific cell body layer distribution manner of one of the tested mice. d, e Fluorescent-labeled neuron proportions of different layers in all cortical brain regions (d) and each cortical brain region (e) of histaminergic neurons. n = 5 (derived from different mice). f Total 150 well reconstructed co-projection structure of upstream cortical neurons in different hemispheres (Top: horizontal view of cortical neurons in left and right hemisphere; Bottom: coronal views of cortical neurons in left and right hemisphere). g, h Co-projection proportion analyze (g) and simplified diagram (h) of well reconstructed neurons in different cortical brain regions. See the list of abbreviations in Supplementary Tables 1 and 2. All data are presented as mean ± SEM.