Fig. 3: Excitatory and inhibitory inputs to histaminergic neurons from major upstream brain regions.
From: A whole-brain male mouse atlas of long-range inputs to histaminergic neurons
a Experimental scheme and timeline for viruses injection and RNAscope in situ hybridization. b–i Representative images of in situ hybridization labeled GABAergic (GAD1) or glutamatergic (Vglut2 or CaMKIIα) markers co-located with RV-eGFP labeled neurons in LS (b), AHY (c), PVT (d), PAG (e), BST (f), CA1 (g), PA/BMAp (h), and MEA (i). j Quantification of eGFP-labeled neurons in major upstream brain regions that are GABAergic (GAD1) or glutamatergic (Vglut2 or CaMKIIα). n = 4. All data are presented as mean ± SEM. All experiments in Fig. 4b–i were repeated independently with similar results for four times. AHY Anterior hypothalamus, BMA Basomedial amygdalar nucleus, BST Bed nuclei of the stria terminalis, CA1 field CA1, LS Lateral septal nucleus, MEA Medial amygdalar nucleus, PA Posterior amygdalar nucleus, PAG Periaqueductal gray, PVT Paraventricular nucleus of the thalamus.
