Fig. 1: GD2TIF cells persist in vivo long-term in a polyclonal state.
From: GD2TIF cells as a platform for single-dose and long-term delivery of biologics
a One million GD2TIF cells were intravenously transferred into male C57BL/6 mice, and their persistence in peripheral blood was monitored over time through serial bleeding (n = 5). b, c FACS analysis of CD62L expression on splenic GD2TIF cells at 2 months post-transfer. Representative plot (b) and quantification (c) are shown (n = 6). d Uniform Manifold Approximation and Projection (UMAP) of 10,290 single GD2TIF cells pooled from 4 mice. e Normalized expression levels of key markers across identified clusters. f Clustered heatmap showing representative marker genes in annotated CD62Lhi and CD62Llo populations. g–i Gene Set Enrichment Analysis (GSEA) comparing CD62Lhi and CD62Llo populations. Exact P values: g 3.64 × 10-7; h 2.10 × 10-5; i 6.45 × 10-11. j Clonotype distribution of GD2TIF cells based on scTCR-seq. Individual clones were identified by unique CDR3 sequences, and the percentages of cells with identical CDR3 sequences among all sequenced cells were calculated. k UMAP visualization of GD2TIF cells, with individual TCR clonotypes distinguished by color. l Distribution of the top three clonotypes across clusters in the UMAP plot, with all other clonotypes grouped as “common clone”. Data represent mean ± s.e.m. from one of three independent experiments. n indicates the number of mice. Exact P values were determined by two tailed paired Student’s t test in (c), and one-sided permutation tests in (g–i), as implemented in the clusterProfiler package (v4.4.4). Source data are provided as a Source Data file.
