Fig. 2: GD2T_IF cells secreting inflammatory cytokines rapidly establish disease models in mice.

a Experimental design for generating GD2T_IF-TNF cells. One million GD2T_IF cells and GD2T_IF-TNF cells were transferred into six-week-old female C57BL/6 mice, which were subsequently monitored for GD2T_IF-TNF cell persistence and monocyte expansion. GFP: green fluorescent protein; 2 A: P2A autoclevage peptide sequence; TNF: tumor necrosis factor alpha. b, c FACS analysis of GD2T_IF-TNF cells in peripheral blood at 4 weeks post-transfer. Representative plot (b) and quantification (c) are shown (n = 3). d–f FACS analysis of CD11b⁺ myeloid cells and Thy1.1⁺ GD2T_IF-TNF cells in peripheral blood. Representative plot (d) and quantification (e, f) are shown (n = 3). g, Body weight changes in mice transferred with GD2T_IF-TNF cells or PBS over 5 weeks post-transfer (n = 3). h, Experimental design for generating GD2T_IF-IL-5 cells. PBS or GD2T_IF-IL-5 cells were transferred into six-week-old female C57BL/6 mice, which were monitored for GD2T_IF-IL-5 cell persistence and eosinophil expansion. IL-5: interleukin-5. i, j, FACS analysis of GD2T_IF-IL-5 cells in peripheral blood at 4 weeks post-transfer. Representative plot (i) and quantification (j) are shown (n = 4). k–m FACS analysis of SiglecF⁺ eosinophils and Thy1.1⁺ GD2T_IF-IL-5 cells in peripheral blood. Representative plot (k) and quantification (l, m) are shown (n = 4). Data represent mean ± s.e.m. from one of two independent experiments. n indicates the number of mice. Exact P values were determined by two tailed unpaired Student’s t test in (c, j) and two-way ANOVA with Bonferroni’s post hoc test in (e–g, l, m). Source data and exact P values are provided as a Source Data file.