Fig. 1: Identification and cloning of SW14 locus in soybean.
From: Natural allelic variation in SW14 determines seed weight and quality in soybean
GWAS scan for seed weight using data from the 320-accession panel grown over the 2018 (a) and 2019 (b) field seasons in Guangzhou (113°23′E, 23°16′N), China. Arrows indicate significant association loci for seed weight across the 2 years. The dashed horizontal lines represent the significance threshold determined by the Bonferroni correction. c QTL mapping of SW14 locus in the F2 segregation population derived from the cross between Yunchun 2014 carrying SW14H1 and Huachun 8 carrying SW14H3. The genetic distance (cM) and the name of markers of the linkage group is given on the left- and the right-hand side of the chromosome model, respectively. d Delimitation of the SW14 locus to a 383-kb region. Graphical genotypes of 7 recombinants carrying crossovers in the SW14 region (left panel), and phenotypic segregation patterns of progeny are shown in boxplot format (right panel), where the box representing the interquartile range, the central line indicating the median, and the whiskers showing the minimum or maximum value (n = 20, one plot indicates one plant). e Heat map of candidate genes located in the candidate region. The color key (blue to red) represents gene expression (fragments per kilobase per million mapped reads, FPKM). f Haplotypes detected in the coding region of SW14. g Comparison of seed weight between different haplotypes of SW14 over the 2018 and 2019 field seasons in Guangzhou, China. In the violin plot, the dashed lines indicate the median and dotted lines the interquartile range. Different lowercase letters indicate statistically significant differences (one-way ANOVA, P < 0.01). Source data are provided as a Source Data file.
