Fig. 4: SW14 interacts with GmLEC1a and GmLEC1b.
From: Natural allelic variation in SW14 determines seed weight and quality in soybean
a Yeast two-hybrid assays showing the interactions of SW14 protein with GmLEC1a and GmLEC1b. Transformed yeast cells were grown on SD/-LW (lacking Leu and Trp) and -LWHA (lacking Leu, Trp, His, and Ade) medium. b, c Split-LUC complementation imaging assay showing the interaction of SW14 with GmLEC1a and GmLEC1b in N. benthamiana leave. GUS-nLUC and cLUC-GUS served as negative controls. d, e Co-IP assay showing the interaction of SW14 with GmLEC1a and GmLEC1b in N. benthamiana leave. The BFP-GFP protein was used as a negative control. The precipitated proteins were detected by α-FLAG or α-GFP antibody. f Seed phenotypes of Tianlong 1 (TL1) and the Gmlec1a mutant lines in Sanya field. Scale bar, 1 cm. Statistical analysis of the 100-seed weight (g), oil content (h), and protein content (i) of TL1 and the Gmlec1a mutant lines. Values represent means ± SD (n = 8, one plot indicates one plant). Transcript levels of FA9 (j) and GmFAD3c (k) in TL1 and the Gmlec1a mutant lines. GmACT11 was used as an internal control. Values represent means ± SD (n = 3 biological replicates). Different lowercase letters indicate statistically significant differences (one-way ANOVA, P < 0.01). Source data are provided as a Source Data file.
