Fig. 5: SW14 inhibits GmLEC1 function in the regulation of seed weight and quality.
From: Natural allelic variation in SW14 determines seed weight and quality in soybean
a Schematic diagram depicts the constructs used in the transient expression assay shown in (b). b Transient expression assay indicating that SW14 inhibits the GmLEC1a/GmNF-YC2/GmbZIP67-mediated activation of FA9 and GmFAD3c promoters. LUC, luciferase; REN, Renilla LUC. A GUS fragment was used as a negative control. Values represent means ± SD (n = 3 biological replicates). Different lowercase letters indicate statistically significant differences (one-way ANOVA, P < 0.05). c Schematic representations of FA9 and GmFAD3c promoters. The regions analyzed by ChIP-qPCR are indicated. Red lines indicate the regions that were bound by GmLEC1 according to previous ChIP-seq data23. d ChIP analysis of GmLEC1a-FLAG binding to the FA9 and GmFAD3c promoters in W82 and sw14#1 mutant. The enrichment of a GmELF3b genomic fragment was used as a negative control. Values represent means ± SD (n = 3 biological replicates). e SW14 inhibits the formation of GmLEC1a/GmNF-YC2/GmbZIP67 complex in yeast. Transformed yeast cells were grown on SD/-LW and -LWHA medium. f Seed phenotypes of TL1, Gmlec1a#1, sw14TL, and Gmlec1a#1 sw14TL mutant lines in Guangzhou field. Scale bar, 1 cm. Statistical analysis of the 100-seed weight (g), oil content (h), and protein content (i) in (f). Values represent means ± SD (n = 6, one plot indicates one plant). Different lowercase letters indicate statistically significant differences (one-way ANOVA, P < 0.01). Source data are provided as a Source Data file.
