Fig. 7: ACSL5-driven fatty acid synthesis suppresses histone acetylation and tumor growth. | Nature Communications

Fig. 7: ACSL5-driven fatty acid synthesis suppresses histone acetylation and tumor growth.

From: SOX2 drives esophageal squamous carcinoma by reprogramming lipid metabolism and histone acetylation landscape

Fig. 7

a The expression profiling and expression correlation analysis of SOX2 and ACSL5 in ESCC tumors from TCGA database. Box plots showing distributions of the normal group (n = 11) and tumor group (n = 95). Box limits indicate IQR, midline represents median. Student’s t test (unpaired, two-tailed). Source data are provided as a Source Data file. b Western blot showing the effect of SOX2 knockdown and ACSL5 ectopic overexpression on histone acetylation in K450 cells. Antibodies against SOX2, ACTIN, ACSL5, H3 were probed on the same gel. The samples originate from the same experiment, FLAG, H3ac and H4ac were processed on separate gels in parallel. c Analysis of the cell proliferation in SOX2 knockdown and ACSL5 overexpressing K450 cells by CCK8 assay. d–f Subcutaneous tumor xenograft assay evaluating the tumorigenic activities of control and SOX2 knockdown K450 cells in nude mice. The tumor growth curves (d), the image of dissected xenografted tumors (e), and the weights of xenografted tumors (f) of shVector-infected and shSOX2 -infected K450 cell xenografts were shown. g–i Subcutaneous tumor formation assay evaluating the tumorigenic activities of control and ACSL5 overexpressing K450 cells. The tumor growth curves (g), the image of dissected xenografted tumors (h), and the weights of xenografted tumors (i) were shown. j Oil-red staining showing the levels of lipid droplets in xenograft tumors derived from representative shVec control, shSOX2 knockdown, OE-Vec, and OE-ACSL5 K450 cell-derived xenograft tumors. Scale bar 100 μm. k IHC staining showing the levels of acetylated H3 or H4 in xenograft tumors derived from shVec control, shSOX2, OE-Vec, and OE-ACSL5 overexpressing K450 cells. Scale bar 50 μm. l RT-PCR analysis showing elevated ACSL5 mRNA level in SOX2 knockdown K450-derived xenograft tumors. RT-PCR data (l) and CCK8 assay data (c) are represented as mean +/- SD from 3 biological replicates, n = 3. Tumor Volume and weight assay (d, g, f, i) data are represented as mean +/- SD, n = 8 (shSOX2 group n = 6). *P  <  0.05, **P  <  0.01, ***P  <  0.001. Student’s t test (unpaired, two-tailed). Source data are provided in the Source Data file.

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