Fig. 3: ISC-specific Pvr knockdown suppresses the migratory response to epithelial damage.

a–e Guts with ISC-specific expression of YFP to label ISCs (in green) and LiveAct-RFP to monitor actin dynamics (in red) dissected from uninfected flies (a–a”, control) or flies exposed to P.e. for 2 h (b–e”). Representative time points from real time recordings showing that ISC-specific Pvr knockdown (c–d”) suppresses ISC migration and the formation of ISC clusters (b–b”), as quantified in (e) by counting the number of cells per cluster (n = 17, 17, 20, 20 guts). The acquired movies were aligned in Fiji using “rigid body transformation to reduce movement related to muscle contractions. f–i The TransTimer system was used to follow the differentiation of ISCs into progenitors and mature cells from 12 to 17 h after infection. At 12 h only a few ISCs (red and green) have differentiated into EBs/EECs/ECs (red only, labeled by while stars), while 5 h later many more ISCs have either differentiated or are in the process of transitioning (more red than yellow) as depicted in (i, n = 5 guts). Significance was tested with Kruskal–Wallis with post-hoc multiple comparison analysis. Data are presented as mean values ± SD. Source data are provided as a Source data file.