Fig. 2: Disruption of ER protein homeostasis inhibits food digestion. | Nature Communications

Fig. 2: Disruption of ER protein homeostasis inhibits food digestion.

From: The shutdown of food digestion due to endoplasmic reticulum homeostasis disruption acts as a protective mechanism in C. elegans

Fig. 2

a Developmental progression of N2, xbp-1(tm2482), xbp-1(zc12), atf-6(ok551) and pek-1(ok275) animals grown on heat-killed E. coli + S. saprophyticus at 20 °C for 4 days. Scale bar, 200 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. b Microscope images and quantification of GFP expression in wild-type and xbp-1(zc12) mutants carrying the hsp-4p::gfp reporter, with L1-stage animals fed on heat-killed E. coli OP50 for 24 h. Scale bar, 50 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. c Representative images and quantification of YFP expression in the CPL-1(W32A Y35A)::YFP reporter strain at day 1 of adulthood, treated with control or xbp-1 RNAi. Scale bar, 200 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. For all panels, n = number of animals which were scored. Representative data shown is one of three biological replicates. Source data are provided as a Source Data file.

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