Fig. 4: FDR-1 is expressed in the intestine and induced by food.

a Bright-field and fluorescence images showing the expression pattern of fdr-1 in transgenic animals carrying fdr-1p::gfp. Scale bar, 100 μm (up), 10 μm (down). b Bright-field and fluorescence images displaying the expression pattern of fdr-1 in transgenic animals with a single-copy insertion of fdr-1p::fdr-1::gfp::flag, constructed via CRISPR/Cas9. Scale bar, 100 μm (left), 10 μm (right). c Microscope images showing co-localization of FDR-1::GFP with the ER marker (vha-6p::SEL-1(1-79)::mCherry::HDEL). Scale bar, 10 μm. d Microscope images and bar graph illustrating GFP expression in fdr-1p::fdr-1::gfp::flag transgenic L1-stage animals after 24 h of feeding with different food sources (E. coli, heat-killed E. coli, heat-killed E. coli + S. saprophyticus, or S. saprophyticus). NGM without food was used as a control. Scale bar, 10 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. e Western blot analysis of FDR-1 protein levels in fdr-1p::fdr-1::gfp::flag transgenic L1-stage animals after 24 h of feeding with different food sources (E. coli, heat-killed E. coli, heat-killed E. coli + S. saprophyticus, or S. saprophyticus). NGM without food served as a control. The relative intensity of FDR-1::GFP::FLAG versus tubulin is quantified from four biological replicates and shown. Mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, via two-sided t-test. For all panels, n = number of animals which were scored. Representative data shown is one of three biological replicates. Source data are provided as a Source Data file. See also Supplementary Figs. 5 and 6.