Fig. 5: XBP-1 regulates FDR-1 expression to maintain ER homeostasis and support food digestion. | Nature Communications

Fig. 5: XBP-1 regulates FDR-1 expression to maintain ER homeostasis and support food digestion.

From: The shutdown of food digestion due to endoplasmic reticulum homeostasis disruption acts as a protective mechanism in C. elegans

Fig. 5

a Schematic illustration depicting the potential relationship between XBP-1 and FDR-1 in maintaining ER homeostasis and regulating food digestion. b Representative images and quantification of GFP expression in CPL-1(W32A Y35A)::YFP transgenic animals at day 1 of adulthood, following treatment with control or fdr-1 RNAi. Scale bar, 100 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. c Representative images and quantification of GFP expression in wild-type and mutant animals carrying the hsp-4p::gfp reporter, with L1-stage animals fed on heat-killed E. coli for 24 h. Scale bar, 20 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. d qRT-PCR analysis showing the expression levels of xbp-1 and xbp-1s in wild-type N2 and fdr-1 mutants, with L1-stage animals fed on E. coli for 24 h. Mean ± SD; ** P < 0.01, *** P < 0.001, via two-sided t-test. e qRT-PCR analysis showing the expression level of C49C8.5 in wild-type N2 and xbp-1 mutants, with L1-stage animals fed on heat-killed E. coli for 24 h. Mean ± SD; ****p < 0.0001 via two-sided t-test. f Microscope images and bar graph showing GFP expression in wild-type and xbp-1(tm2482) mutants carrying fdr-1p::gfp, with L1-stage animals fed on heat-killed E. coli for 24 h. Scale bar, 20 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. g Microscope images and bar graph showing GFP expression in wild-type and xbp-1(tm2482) mutants carrying fdr-1p::fdr-1::gfp, with L1-stage animals fed on heat-killed E. coli for 24 h. Scale bar, 20 μm. Mean ± SD; ****p < 0.0001 via two-sided t-test. h Developmental progression of N2, fdr-1(ok3369), xbp-1(zc12), and fdr-1(ok3369);xbp-1(zc12) animals grown on heat-killed E. coli + S. saprophyticus at 20 °C for 4 days. Scale bar, 200 μm. Mean ± SD; ns denotes p > 0.05,****p < 0.0001 via two-sided t-test. For all panels, n = number of animals which were scored. Representative data shown is one of three biological replicates. Source data are provided as a Source Data file. See also Supplementary Fig. 7.

Back to article page