Fig. 1: HCS-3DX, an AI-driven HCS system for 3D imaging and analysis of 3D-oids at a single-cell level.
From: HCS-3DX, a next-generation AI-driven automated 3D-oid high-content screening system
a HCS-3DX workflow includes 8 essential steps: (I) 3D cell culture generation, (II) AI-driven 2D object detection, feature analysis and selection, (III) automated sample transfer from the source culture vessel, (IV) transfer to a custom-developed HCS imaging plate, (V) LSFM screening, (VI) generation of massive 3D image datasets, (VII) image analysis with BIAS, (VIII) feature extraction and single-cell classification. b Step II-IV - working principle of the SpheroidPicker, an AI-guided, 3D cell culture delivery system using a light microscope for 2D morphology-based spheroid screening and analysis, enabling semi- or fully-automated transfer of selected spheroids with predefined morphology features. c Step IV - selected spheroids are transferred to the HCS foil multiwell plate compatible with LSFM to obtain single-cell resolution in 3D. d Step VII - a representative image of a nuclei-labelled spheroid analysed and visualised by BIAS, with single cells indicated by green bounding boxes.
