Fig. 7: Comparison of spherical and irregular spheroids.
From: HCS-3DX, a next-generation AI-driven automated 3D-oid high-content screening system
a Vertical projection of a spherical and irregular spheroid that displays the number of HeLa Kyoto (purple) and MRC-5 (orange) cells. The centre of each spheroid was defined using whole spheroid segmentation. Starting from the centre position, spheres with increasing radius were used to create shells and count the number of objects in each section. Line plots show the number of cells per class based on the shell analysis. b Spider plot representation of the Spearman’s correlation between 2D and 3D features. Paired spheroids ranked by Solidity 3D (grey, n = 34) are divided into a Spherical (dark blue, n = 18) and Irregular (red, n = 16) groups. c Correlation between 2D and 3D features (n = 34). Previously displayed spherical (dark blue) and irregular (red) spheroid, along with the paired samples are displayed. Outliers (black and red) were not used for the evaluation. All scatter plots were generated using a fitted regression line, with shaded bands indicating the standard error of the mean (SEM). Source data are provided as a Source Data file.
