Fig. 8: Comparative representation of a regular and irregular 3D sample.
From: HCS-3DX, a next-generation AI-driven automated 3D-oid high-content screening system
a 3D visualisation of the Spherical and Irregular spheroids where each circle represents a cell displayed based on their position within the spheroid and class (HeLa Kyoto - purple and MRC-5 - orange). Each class is also visualised separately. b Collected 2D, 3D, and Neighbourhood (NH) features for both spheroids are displayed. c 3D representation of the Spherical and Irregular co-culture spheroids. Both top and side views show the nuclei of the HeLa Kyoto (purple) and MRC-5 (orange) cells. For visualisation, a square was placed around the spheroids indicating the side view. The scale bar represents 50 µm for the top and 100 µm for the side view. Leica’s LAS X microscope software was used to display the images.
