Fig. 2: Validation of MlIW39 candidate genes using mutagenesis.
From: Two complementary NLRs from wild emmer wheat confer powdery mildew resistance
a Powdery mildew responses of 8D49, Zavitan and five susceptible mutants at 15 days post-inoculation (dpi) with Bgt isolate E09. b Predicted structures of the NLR-1 and NLR-2 genes and locations of the independent mutations. Gray rectangles indicate coding exons. Positions of the mutations are indicated by black lines. The coding sequence (c.) changes and their predicted effects on the translated protein (p.) are indicated below the mutants and Zavitan. c, d Three-dimensional (3D) models of NLR-1 and NLR-2 predicted by AlphaFold 3. The red spheres indicate amino acid substitutions from EMS mutagenesis and from Zavitan. Source data are provided as a Source Data file.
