Fig. 4: MlIW39-R1^CC self-associates and interacts with MlIW39-R2^CC-like.

a Cell death response in N. benthamiana triggered by co-expression of MlIW39-R1 and MlIW39-R2, whereas MlIW39-R1 or MlIW39-R2 alone did not. The Autofluorescence indicative of cell death under ultraviolet light is shown. Protein expression was detected by immunoblotting, and Ponceau S staining of Rubisco served as a loading control. Experiments were performed twice with similar results. b–e Protein-protein interaction between the CC domains of MlIW39-R1 was detected by Y2H, Luc, BiFC and Co-IP assays. AD, activation domain; BD, binding domain; Positive control (53 + T); Negative control (empty vectors AD + BD). The cLUC-TaSUMO1+nLUC-TaHsfA1 were used as a positive control in the Luc assay. Experiments were performed twice with similar results. f, g Interaction between MlIW39-R1^CC and MlIW39-R2^CC-like was confirmed by Y2H and Co-IP assays. Experiments were performed twice with similar results. Source data are provided as a Source Data file.