Fig. 5: Single-nucleus transcriptome analyses of NR3C1 cKO mice at the peak of EAE.
From: NR3C1-mediated epigenetic regulation suppresses astrocytic immune responses in mice
a Heatmap representing DEGs between astrocytes from Cre− control and NR3C1 cKO mice at the EAE peak. b t-SNE projections illustrating subpopulation information of astrocytes (C0-C4). c Dot plots showing the expression patterns of DEGs in astrocyte subpopulations. Dot size indicates the average ratio of expressed gene numbers per cell. Color represents the average expression level of genes in subpopulations. d Scatter plots of representative DEG expressions in astrocytes from Cre− control and NR3C1 cKO mice. e Violin plots showing expression levels of DEGs at the EAE peak in active or inactive brain lesions of multiple sclerosis patients (two-sided Kolmogorov–Smirnov test, Up, P < 2.20 × 10−16; Down, P < 2.20 × 10−16). f Top 5 enriched MSigDB Hallmark pathways of upregulated or downregulated DEGs in astrocytes from NR3C1 cKO mice at the EAE peak. P values were calculated by one-sided hypergeometric test. g Violin plots showing gene expression scores of TNF-α signaling (P = 2.89 × 10−15), IFN-γ signaling (P = 0.015), and IL-2/STAT5 signaling (P = 8.77 × 10−5). The box indicates the IQR, and the whiskers represent the highest and lowest points within 1.5 × IQR. h Distribution of fold change values between Cre− control and NR3C1 cKO for GR-suppressing LPS responsive genes (P = 9.66 × 10−15). P values were calculated by two-sided Kolmogorov–Smirnov test.
